DNA Extracted from Stained Sputum Smears Can Be Used in the MTBDR plus Assay
نویسندگان
چکیده
منابع مشابه
DNA extracted from stained sputum smears can be used in the MTBDRplus assay.
We examined the feasibility of using DNA extracted from stained sputum smears for the detection of rifampin and isoniazid resistance with the commercial MTBDRplus assay from Hain Lifescience GmbH, Nehren, Germany. Overall sensitivity was initially low (70.0%) but increased to 96.7% when a multiplex PCR preamplification step was added. We then tested stored Mycobacterium tuberculosis-positive st...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2011
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00745-11